SOP 5: Modified Knott’s Test

Equipment

  • 2% formalin (diluted in distilled water)
  • 0.1% Methylene blue
  • Centrifuge
  • 15 ml centrifuge tube

Method

  • 1 ml blood is lysed by mixing with 10 ml of 2% formalin
  • Centrifuge at 1500 rpm for 5 min to deposit microfilaria and erythrocyte and white cell walls at the       bottom of the tube
  • Discard supernatant
  • Stain sediment for 1-2 min with 1-2 drops of 0.1% methylene blue and examine as a wet mount
  • Examine for microfilaria

Safety Precautions

  • Wear lab coat and disposable gloves

Clean Up Procedures

  • Dispose of all slides and cover slips in a sharps container